Lack of exon 10 in the murine tau gene results in mild sensorimotor defects with aging

BMC Neuroscience

Lars NG Nilsson¹  Lars Lannfelt²  Astrid Gumucio² 

[1] Department of Pharmacology, Oslo University and Oslo University Hospital, Postboks 1057 Blindern, NO-0316 Oslo, Norway;Department of Public Health and Caring Sciences, Rudbeck Laboratory, Uppsala University, SE-75185 Uppsala, Sweden

关键词: Microtubule;    Alternative splicing;    Knockout mice;    Tau;    Alzheimer’s disease;   

Others  :  1131187
DOI  :  10.1186/1471-2202-14-148

 received in 2013-08-21, accepted in 2013-11-19,  发布年份 2013

【 摘 要 】

Background

Complex species-specific, developmental- and tissue-dependent mechanisms regulate alternative splicing of tau, thereby diversifying tau protein synthesis. The functional role of alternative splicing of tau e.g. exon 10 has never been examined in vivo, although genetic studies suggest that it is important to neurodegenerative disease.

Results

Gene-targeting was used to delete exon 10 in murine tau on both alleles (E10−/−) to study its functional role. Moreover, mice devoid of exon 10 (E10+/−) on one allele were generated to investigate the effects of 1:1 balanced expression of 4R-/3R-tau protein, since equal amounts of 4R-/3R-tau protein are synthesized in human brain. Middle-aged E10−/− mice displayed sensorimotor disturbances in the rotarod when compared to age-matched E10+/− and wild-type mice, and their muscular grip strength was less than that of E10+/− mice. The performance of E10+/− mice and wild-type mice (E10+/+) was similar in sensorimotor tests. Cognitive abilities or anxiety-like behaviours did not depend on exon 10 in tau, and neither pathological inclusions nor gene-dependent morphological abnormalities were found.

Conclusion

Ablation of exon 10 in the murine tau gene alters alternative splicing and tau protein synthesis which results in mild sensorimotor phenotypes with aging. Presumably related microtubule-stabilizing genes rescue other functions.

【 授权许可】

   
2013 Gumucio et al.; licensee BioMed Central Ltd.

【 图 表 】

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