A 22 kDa polyanion inhibits carnitine‐dependent fatty acid oxidation in rat liver mitochondria

FEBS Letters

Hoppel, Charles¹  Kerner, Janos¹  Turkaly, Peter¹ 

[1] Department of Veterans Affairs Medical Center, Departments of Nutrition, Medicine and Pharmacology, Medical Research Service (151W), 10701 East Blvd., Case Western Reserve University, Cleveland, OH 44106, USA

关键词: Mitochondrion;    Carnitine;    Fatty acid oxidation;    Porin;    Polyanion;    Carnitine palmitoyltransferase;    VDAC;    voltage-dependent anion channel (porin);    CPT-I;    malonyl-CoA sensitive carnitine palmitoyltransferase;    PA-22;    22 kDa polyanion;   

DOI  :  10.1016/S0014-5793(99)01354-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The transport of activated fatty acids across the mitochondrial outer membrane has not been fully addressed. A polyanion (M _n=22 kDa) inhibited the ADP-stimulated carnitine-dependent oxidation of both palmitoyl-CoA and palmitate plus CoA as well as mitochondrial hexokinase binding. In contrast, the oxidation of palmitoylcarnitine plus malate, as well as glutamate oxidation, was essentially unaffected. Mitochondrial carnitine palmitoyltransferase-1 was not inhibited by the polyanion. The data suggest an additional component in carnitine-dependent mitochondrial fatty acid oxidation, possibly porin.

【 授权许可】

Unknown