Identification of residues involved in binding of IL5 to βcom using βIL3 and βcom chimeras

FEBS Letters

Sanderson, Colin J¹  Czabotar, Peter E¹  Holland, John² 

[1] Division of Molecular Immunology, School of Biomedical Sciences, Curtin University of Technology, Level 5 MRF Building, Rear 50 Murray Street, Perth, W.A. 6000, Australia;Urological Research Centre, QEII Medical Centre, Nedlands, W.A. 6009, Australia

关键词: Interleukin 5;    Interleukin 3;    Cytokine receptor;    βcom;    βIL3;   

DOI  :  10.1016/S0014-5793(99)01323-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In mice there are two forms of the beta chain used in the IL3 receptor system, β_com and β_IL3. β_com is used by the IL3, IL5 and GM-CSF receptors whereas β_IL3 is only used in the IL3 receptor. In this work an assay was developed to identify residues of β_IL3 that restrict IL5 activity. It was found that such residues reside within the 2nd CRM of the molecule. Furthermore, when residues in the β_IL3 B′-C′ loop were replaced with β_com sequence a form of β_IL3 was produced that was able to respond to IL5. This region is also responsible for IL3 binding to β_IL3 in the absence of alpha chain. It is therefore an important structural motif of β_com and β_IL3 responsible for both ligand interaction and specificity.

【 授权许可】

Unknown