Direct evidence of cytoplasmic delivery of PKC‐α, ‐ϵ and ‐ζ pseudosubstrate lipopeptides: study of their implication in the induction of apoptosis

FEBS Letters

Hodges, Robin²  Verwaerde, Claudie¹  Gras-Masse, Hélène³  Loing, Estelle³  Rommens, Corinne³  Zoukhri, Driss²  Thiam, Kader¹  Sergheraert, Christian³  Auriault, Claude¹  Dartt, Darlene² 

[1] UMR 8727, Lille II University, Institut de Biologie et Institut Pasteur de Lille, 1 rue du Pr. A. Calmette, P.O. Box 447, 59021 Lille, France;Department of Ophtalmology, Schepens Eye Research Institute, Harvard Medical School, 20 Staniford Street, Boston, MA 02114, USA;UMR 8525, Lille II University, Institut de Biologie et Institut Pasteur de Lille, 1 rue du Pr. A. Calmette, P.O. Box 447, 59021 Lille, France

关键词: PKC pseudosubstrate lipopeptide;    Intracellular delivery;    PKC isoform;    Apoptosis;   

DOI  :  10.1016/S0014-5793(99)01240-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein kinases C (PKC) are serine/threonine kinase enzymes involved in the mechanism of cell survival. Their pseudosubstrate sequences are autoinhibitory domains, which maintain the enzyme in an inactive state in the absence of allosteric activators, thus representing an attractive tool for the modulation of different PKC isoforms. Here, we report the use of palmitoylated modified PKC-α, -ϵ, and -ζ pseudosubstrate peptides, and determine their intracellular distribution together with their respective PKC isoenzymes. Finally, we propose that the differential distribution of the peptides is correlated with a selective induction of apoptosis and therefore argues for different involvement of PKC isoforms in the anti-apoptotic program.

【 授权许可】

Unknown