Evidence for a role of C‐terminus in Ca2+ inactivation of skeletal muscle Ca2+ release channel (ryanodine receptor)

FEBS Letters

Pasek, Daniel A.²  Xin, Chunlin²  Gao, Ling²  Xu, Le²  Nakai, Junichi¹  Meissner, Gerhard² 

[1] Department of Information Physiology, National Institute for Physiological Sciences, Myodaiji-cho, Okazaki 444-8585, Japan;Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599-7260, USA

关键词: Ca2+ release channel;    Ryanodine receptor;    Skeletal muscle;    Cardiac muscle;    HEK293 cell;    HEK;    human embryonic kidney;    E-C;    excitation-contraction;    RyR1;    skeletal muscle Ca2+ release channel (ryanodine receptor);    RyR2;    cardiac muscle Ca2+ release channel (ryanodine receptor);    SR;    sarcoplasmic reticulum;    DHPR;    dihydropyridine receptor;    P o;    channel open probability;   

DOI  :  10.1016/S0014-5793(99)01232-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Six chimeras of the skeletal muscle (RyR1) and cardiac muscle (RyR2) Ca²⁺ release channels (ryanodine receptors) previously used to identify RyR1 dihydropyridine receptor interactions [Nakai et al. (1998) J. Biol. Chem. 273, 13403] were expressed in HEK293 cells to assess their Ca²⁺ dependence in [³H]ryanodine binding and single channel measurements. The results indicate that the C-terminal one-fourth has a major role in Ca²⁺ activation and inactivation of RyR1. Further, our results show that replacement of RyR1 regions with corresponding RyR2 regions can result in loss and/or reduction of [³H]ryanodine binding affinity while maintaining channel activity.

【 授权许可】

Unknown