FEBS Letters | |
Functional analysis of rat renal organic anion transporter OAT‐K1: bidirectional methotrexate transport in apical membrane | |
Masuda, Satohiro1  Takeuchi, Ayako1  Inui, Ken-ichi1  Saito, Hideyuki1  Hashimoto, Yukiya1  | |
[1] Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan | |
关键词: Organic anion transporter; Methotrexate; Brush-border membrane; Tubular secretion; MDCK cell; (Rat kidney); RT; reverse transcription; PCR; polymerase chain reaction; oatp; organic anion transporting polypeptide; | |
DOI : 10.1016/S0014-5793(99)01221-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Renal organic anion transporter OAT-K1 was stably transfected in MDCK cells and examined for its transport characteristics and membrane localization. OAT-K1 mediated both uptake and efflux of methotrexate in the apical membranes. Immunoblotting showed that the apparent molecular mass of the expressed OAT-K1 was 50 kDa, which was comparable to that found in the rat renal brush-border membranes. The OAT-K1-mediated methotrexate transport was significantly inhibited in the presence of several organic anions such as folate and sulfobromophthalein. These findings suggest that OAT-K1 mediates bidirectional methotrexate transport across the apical membranes, and may be involved in the renal handling of methotrexate.
【 授权许可】
Unknown
【 预 览 】
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RO201912020308369ZK.pdf | 179KB | download |