Cloning of an α‐glucosidase gene from Thermococcus hydrothermalis by functional complementation of a Saccharomyces cerevisiae mal11 mutant strain

FEBS Letters

Galichet, Arnaud¹  Belarbi, Abdel¹ 

[1] Laboratoire de Microbiologie Générale et Moléculaire, UFR Sciences, Université de Reims, P.O. Box 1039, 51687 Reims Cedex 2, France

关键词: α-Glucosidase;    Thermophilic archaeon;    mal11 mutant;    Thermococcus hydrothermalis;    Saccharomyces cerevisiae;   

DOI  :  10.1016/S0014-5793(99)01155-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α-Glucosidase is found in methanogenic and thermophilic archaea and also in eukaryotes and bacteria. The gene encoding the enzyme was cloned from Thermococcus hydrothermalis by complementation of a Saccharomyces cerevisiae deficiency maltase mutant strain. The gDNA clone isolated encodes an open reading frame corresponding to a protein of 242 amino acids. The protein shows 42% identity to a Pyrococcus horikoshii unknown ORF but no similarities were obtained with polysaccharidase sequences.

【 授权许可】

Unknown