期刊论文详细信息
FEBS Letters | |
Cloning of an α‐glucosidase gene from Thermococcus hydrothermalis by functional complementation of a Saccharomyces cerevisiae mal11 mutant strain | |
Galichet, Arnaud1  Belarbi, Abdel1  | |
[1] Laboratoire de Microbiologie Générale et Moléculaire, UFR Sciences, Université de Reims, P.O. Box 1039, 51687 Reims Cedex 2, France | |
关键词: α-Glucosidase; Thermophilic archaeon; mal11 mutant; Thermococcus hydrothermalis; Saccharomyces cerevisiae; | |
DOI : 10.1016/S0014-5793(99)01155-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
α-Glucosidase is found in methanogenic and thermophilic archaea and also in eukaryotes and bacteria. The gene encoding the enzyme was cloned from Thermococcus hydrothermalis by complementation of a Saccharomyces cerevisiae deficiency maltase mutant strain. The gDNA clone isolated encodes an open reading frame corresponding to a protein of 242 amino acids. The protein shows 42% identity to a Pyrococcus horikoshii unknown ORF but no similarities were obtained with polysaccharidase sequences.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020308295ZK.pdf | 232KB | download |