Calcium‐induced calcium release mediated by a voltage‐activated cation channel in vacuolar vesicles from red beet

FEBS Letters

Maathuis, Frans J.M¹  Sanders, Dale¹  Bewell, Michael A¹  Allen, Gethyn J¹ 

[1] The Plant Laboratory, Department of Biology, University of York, P.O. Box 373, York YO10 5YW, UK

关键词: Calcium signaling;    Calcium release;    Slow vacuolar channel;    Membrane vesicle;   

DOI  :  10.1016/S0014-5793(99)01109-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Little is known about the mechanisms underlying calcium-induced Ca²⁺ release (CICR) in plants. The slow-activating vacuolar (SV) channel is both permeable to, and activated by Ca²⁺, and is therefore a prime candidate for a role in CICR. Cytosol-side-out vacuolar membrane vesicles loaded with ⁴⁵Ca²⁺ showed voltage- and Ca²⁺-dependent Ca²⁺ release, which was sensitive to the SV channel modulators DIDS, protein phosphatase 2B and calmodulin. Significantly, voltage-dependent Ca²⁺ release strongly depended on cytoplasmic Ca²⁺ concentrations. The results support the notion that CICR occurs in plant cells and that the process can be catalysed by the SV channel on the vacuolar membrane.

【 授权许可】

Unknown