Inositol 1,4,5‐triphosphate microinjection triggers activation, but not meiotic maturation in amphibian and starfish oocytes

FEBS Letters

Sladeczek, F.¹  Le Bouffant, F.⁴  Giraud, F.³  Picard, A.⁴  Le Peuch, C.⁴  Dorée, M.² 

[1] Centre CNRS-INSERM de Pharmacologie-Endocrinologie, Montpellier Cedex, France;CNRS-ERA 0415 Physiologie de la nutrition. Université de Paris Sud, Orsay, France;Laboratoire Arago, Université Paris VI, Banyuls-sur-Mer, France;INSERM V. 249, CNRS LP 8402 and Faculté de Pharmacie, BP 5015, 34033 Montpellier Cedex, France

关键词: Inositol 1;    4;    5-triphosphate;    Calcium release;    Exocytosis;    Cell division;   

DOI  :  10.1016/0014-5793(85)80351-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Inositol 3,4,5-triphosphate (InsP3) brought about cortical granule exocytosis and elevation of a fertilization membrane, due to a rapid increase of free calcium in cytoplasm, when injected into oocytes of the amphibian Xenopus laevis arrested at second meiotic metaphase. The same result was observed when injection was performed into oocytes of the starfish Marthasterias glacialis arrested either at the first meiotic prophase or after completion of meiosis. Although meiotic maturation was induced in both animals by specific hormones which have been previously shown to release Ca²⁺ within cytoplasm, InsP3 microinjection into prophasearrested oocytes did not release them from prophase block.

【 授权许可】

Unknown