Sphingosylphosphorylcholine induces cytosolic Ca2+ elevation in endothelial cells in situ and causes endothelium‐dependent relaxation through nitric oxide production in bovine coronary artery

FEBS Letters

Mogami, Kimiko³  Kobayashi, Sei¹  Ohmura, Masato³  Yoshida, Kazuki³  Mizukami, Yoichi³  Miwa, Saori³  Matsuda, Masako¹  Todoroki-Ikeda, Natsuko³  Matsuzaki, Masunori² 

[1]School of Allied Health Sciences, Yamaguchi University, 1-1-1 Minamikogushi, Ube 755-8505, Japan

[2]Second Department of Medicine, School of Medicine, Yamaguchi University, 1-1-1 Minamikogushi, Ube 755-8505, Japan

[3]First Department of Physiology, School of Medicine, Yamaguchi University, 1-1-1 Minamikogushi, Ube 755-8505, Japan

关键词: Sphingolipid;    Cytosolic Ca2+;    Nitric oxide;    Vasorelaxation;    2;    3-Diaminonaphthalene;    Endothelial cell;    SPC;    sphingosylphosphorylcholine;    NO;    nitric oxide;    [Ca2+]i;    intracellular Ca2+ concentration;    NOS;    nitric oxide synthase;    iNOS;    inducible NOS;    eNOS;    endothelial NOS;    ATP;    adenosine 5′-triphosphate;    DAN;    2;    3-diaminonaphthalene;    DiI-Ac-LDL;    acetylated low-density lipoprotein labeled with 1;    1′-dioctadecyl-3;    3;    3′;    3′-tetramethyl-indo-carbocyanine perchlorate;    L-NMMA;    N ω-monomethyl-L-arginine;    TRIM;    1-(2-trifluoromethylphenyl) imidazole;    BK;    bradykinin;    PSS;    physiological salt solution;    IP3;    inositol 1;    4;    5-trisphosphate;   

DOI  :  10.1016/S0014-5793(99)01076-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sphingosylphosphorylcholine (SPC) increased intracellular Ca²⁺ concentration ([Ca²⁺]i) and nitric oxide (NO) production in endothelial cells in situ on bovine aortic valves, and induced endothelium-dependent relaxation of bovine coronary arteries precontracted with U-46619. The SPC-induced vasorelaxation was inhibited by N ^ω-monomethyl-L-arginine, an inhibitor of both constitutive and inducible NO synthase (NOS), but not by 1-(2-trifluoromethylphenyl) imidazole, an inhibitor of inducible NOS (iNOS). Immunoblotting revealed that endothelial constitutive NOS, but not iNOS, was present in endothelial cells in situ on the bovine aortic valves. We propose that SPC activates [Ca²⁺]i levels and NO production of endothelial cells in situ, thereby causing an endothelium-dependent vasorelaxation.

【 授权许可】

Unknown