A novel anti‐hypertensive peptide derived from ovalbumin induces nitric oxide‐mediated vasorelaxation in an isolated SHR mesenteric artery

FEBS Letters

Usui, Hachiro¹  Fujita, Hiroyuki¹  Matoba, Nobuyuki¹  Yoshikawa, Masaaki¹ 

[1]Research Institute For Food Science, Kyoto University, Uji, Kyoto 611-0011, Japan

关键词: Ovalbumin;    Ovokinin;    Nitric oxide;    Vasorelaxation;    Blood pressure;    Spontaneously hypertensive rat;    SHR;    spontaneously hypertensive rat;    WKY;    Wistar-Kyoto rat;    L-NAME;    N G-nitro-L-arginine methyl ester;    NO;    nitric oxide;    HPLC;    high performance liquid chromatography;    TFA;    trifluoroacetic acid;    ODS;    octadecyl silica;    i.p.;    intraperitoneal;   

DOI  :  10.1016/S0014-5793(99)00587-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this report, we deal with the isolation of a novel vasorelaxing peptide from a chymotryptic digest of ovalbumin and its vasorelaxing activities. This peptide is composed of Arg-Ala-Asp-His-Pro-Phe (RADHPF) in its sequence, corresponding to residues 359–364 of ovalbumin. This peptide (30–300 μM) exerted a dose-dependent vasodilation in an isolated mesenteric artery from a spontaneously hypertensive rat which was pre-constricted by phenylephrine, besides the relaxation being endothelium-dependent. It is noteworthy that the nitric oxide synthase inhibitor N ^G-nitro-L-arginine methyl ester inhibited this relaxation, implying involvement of nitric oxide in its mechanism of action. Following oral administration of RADHPF at a dose of 10 mg/kg, the systolic blood pressure in a spontaneously hypertensive rat was significantly lowered.

【 授权许可】

Unknown