Bromine K‐edge EXAFS studies of bromide binding to bromoperoxidase from Ascophyllum nodosum

FEBS Letters

Hanss, Jan⁴  Dittmer, Jens²  Schulzke, Carola¹  Vilter, Hans³  Dau, Holger²  Epple, Matthias¹  Rehder, Dieter¹  Kiss, Erzsébet¹ 

[1] Institut für Anorganische und Angewandte Chemie, Universität Hamburg, D-20146 Hamburg, Germany;Fachbereich Biologie, Universität Marburg, D-35032 Marburg, Germany;Zurmaienstraße 16, D-54292 Trier, Germany;Institut für Physik, Universität Augsburg, D-86159 Augsburg, Germany

关键词: Bromoperoxidase;    Bromine K-edge EXAFS;    Bromovanadium complex;    Aqueous rubidium bromide;   

DOI  :  10.1016/S0014-5793(99)01045-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bromine K-edge EXAFS studies have been carried out for bromide/peroxidase samples in Tris buffer at pH 8. The results are compared with those of aqueous (Tris-buffered) bromide and vanadium model compounds containing Br-V, Br-C(aliphatic) and Br-C(aromatic) bonds. It is found that bromide does not coordinate to the vanadium centre. Rather, bromine binds covalently to carbon. A possible candidate is active site serine.

【 授权许可】

Unknown