A biomimetic model for vanadium‐containing bromoperoxidase

FEBS Letters

Tsuchiya, Koichiro¹  Sakurai, Hiromu¹ 

[1] Faculty of Pharmaceutical Sciences, University of Tokushima, Sho-machi 1, Tokushima 770, Japan

关键词: Vanadium;    Bromoperoxidase;    Biomimetic model;    Oxygen consumption;    Electron spin resonance;    Spin-trapping;   

DOI  :  10.1016/0014-5793(90)80079-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A biomimetic model of vanadium-containing bromoperoxidase (BPO) was proposed. The vanadium-containing BPO possesses vanadate ion (+5 oxidation state) as a prosteric group and catalyses the formation of a carbon-bromide bond in the presence of hydrogen peroxide, bromide ion and bromide acceptor. The reaction mechanism is as yet unknown. Several vanadyl (VO²⁺, +4 oxidation state) complexes with oxalate, glutarate, succinate, malonate and acetate ligands showed similar bromination activity to that of BPO. Especially, the vanadyl-oxalato complex provided the highest BPO-like activity. Both vanadate ion and vanadyl complexes with nitrogen-containing ligands such as EDTA, glycine and serine showed essentially no BPO-like activity. Thus, the vanadyl-oxalato complex is proposed to be not only a possible chemical model of BPO, but a useful compound to elucidate the reaction mechanism of BPO.

【 授权许可】

Unknown