Detergent‐free membrane protein crystallization

FEBS Letters

Nollert, Peter¹  Landau, Ehud M.¹  Pebay-Peyroula, Eva³  Royant, Antoine² 

[1] Department of Molecular Microbiology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland;ESRF, 6 rue Jules Horowitz, P.O. Box 220, F-38043 Grenoble Cedex 1, France;Université Joseph Fourier, Grenoble, France

关键词: Bacteriorhodopsin;    Crystallization;    Lipidic cubic phase;    Membrane protein;    Purple membrane;   

DOI  :  10.1016/S0014-5793(99)01014-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A comprehensive understanding of structure-function relationships of proteins requires their structures to be elucidated to high resolution. With most membrane proteins this has not been accomplished so far, mainly because of their notoriously poor crystallizability. Here we present a completely detergent-free procedure for the incorporation of a native purple membrane into a monoolein-based lipidic cubic phase, and subsequent crystallization of three-dimensional bacteriorhodopsin crystals therein. These crystals exhibit comparable X-ray diffraction quality and mosaicity, and identical crystal habit and space group to those of bacteriorhodopsin crystals that are grown from detergent-solubilized protein in cubic phase.

【 授权许可】

Unknown