Inhibition of protein tyrosine phosphatases blocks calcium‐induced activation of metaphase II‐arrested oocytes of Xenopus laevis

FEBS Letters

Bodart, Jean-François¹  Bertout, Marc¹  Vilain, Jean-Pierre¹  Gannon, Julian²  Rousseau, Arlette¹  Flament, Stéphane¹  Béchard, David¹ 

[1] Centre de Biologie Cellulaire, Laboratoire de Biologie du Développement, UPRES EA 1033, Université de Lille 1, SN3, F-59655 Villeneuve d'Ascq Cedex, France;Imperial Cancer Research Fund Clare Hall Laboratories, South Mimms, Herts., UK

关键词: Oocyte;    Calcium;    Protein tyrosine phosphatase;    Activation;    Xenopus;   

DOI  :  10.1016/S0014-5793(99)00986-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the effect of a protein tyrosine phosphatases (PTP) inhibitor on calcium-induced activation of Xenopus laevis oocytes arrested at metaphase II. Ammonium molybdate microinjection blocked pronucleus formation following A23187 treatment while cortical granules still underwent exocytosis. Pronuclei still occurred in ammonium molybdate-injected oocytes following 6-DMAP addition. Changes that usually occurred following A23187 exposure were inhibited in the presence of ammonium molybdate in the oocyte: MAPK dephosphorylation, p34^cdc2 rephosphorylation and cyclin B2 and p39^mos proteolysis. These results suggest that a PTP is involved in the activation of the ubiquitin-dependent degradation machinery.

【 授权许可】

Unknown