Temperature‐dependent interaction of thermo‐sensitive polymer‐modified liposomes with CV1 cells

FEBS Letters

Nakai, Ryoichi¹  Takagishi, Toru¹  Kono, Kenji¹  Morimoto, Keiji¹ 

[1] Department of Applied Materials Science, College of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Sakai, Osaka 599-8531, Japan

关键词: Temperature-sensitive liposome;    Lower critical solution temperature;    Poly(N-acryloylpyrrolidine);    Poly(N-isopropylacrylamide);    Liposome-cell interaction;    Drug delivery system;    NIPAM;    N-isopropylacrylamide;    APr;    N-acryloylpyrrolidine;    LCST;    lower critical solution temperature;    Poly(APr-co-NIPAM)-2C12;    copolymer of APr and NIPAM having two dodecyl groups at the terminal;    EYPC;    egg yolk phosphatidylcholine;    NBD-PE;    N-(7-nitrobenz-2-oxa-1;    3-diazol-4-yl)phosphatidylethanolamine;    MTX;    methotrexate;    DMEM;    Dulbecco's modified Eagle's medium;    Tris;    tris(hydroxymethyl)aminomethane;    FBS;    fetal bovine serum;    PBS;    phosphate-buffered saline;    PBS-CM;    PBS containing 0.36 mM CaCl2 and 0.42 mM MgCl2;   

DOI  :  10.1016/S0014-5793(99)00975-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Egg yolk phosphatidylcholine liposomes modified with a copolymer of N-acryloylpyrrolidine and N-isopropylacrylamide having a lower critical solution temperature at ca. 40°C were prepared and an effect of temperature on their interaction with CV1 cells was investigated. The unmodified liposomes were taken up by the cells approximately to the same extent after 3 h incubation at 37 and 42°C. In contrast, uptake of the polymer-modified liposomes by CV1 cells decreased slightly at 37°C but increased greatly at 42°C, compared to the unmodified liposomes. Proliferation of the cells was partly prohibited by the incubation with the unmodified liposomes encapsulating methotrexate at 37 and 42°C. The treatment with the polymer-modified liposomes containing methotrexate at 37°C hardly effected the cell growth. However, the treatment at 42°C inhibited the cell growth completely. It is considered that the highly hydrated polymer chains attached to the liposome surface suppressed the liposome-cell interaction below the lower critical solution temperature of the polymer but the dehydrated polymer chains enhanced the interaction above this temperature. Because interaction of the polymer-modified liposomes with cells can be controlled by the ambient temperature, these liposomes may have potential usefulness as efficient site-specific drug delivery systems.

【 授权许可】

Unknown