FEBS Letters | |
Structures of the O‐specific polysaccharides and a serological cross‐reactivity of the lipopolysaccharides of Proteus mirabilis O24 and O29 | |
Knirel, Yuriy A.2  Senchenkova, Sof'ya N.2  Shashkov, Alexander S.2  Kaca, Wieslaw2  Zatonsky, George V.2  Literacka, Elzbieta1  Perepelov, Andrei V.2  | |
[1] Institute of Microbiology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland;N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 117913 Moscow, Russian Federation | |
关键词: O-antigen; Lipopolysaccharide; Bacterial polysaccharide structure; Serological cross-reactivity; Proteus mirabilis; | |
DOI : 10.1016/S0014-5793(99)00952-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Strains of Proteus mirabilis belonging to serogroups O24 and O29 are frequent in clinical specimens. Anti-P. mirabilis O24 serum cross-reacted with the lipopolysaccharide (LPS) of P. mirabilis O29 and vice versa. The structures of the O-specific polysaccharides (OPSs, O-antigens) of both LPSs were established using sugar analysis and one- and two-dimensional 1H- and 13C-NMR spectroscopy and found to be different. SDS-PAGE and Western immunoblotting suggested that the serological cross-reactivity of the LPSs is due to a common epitope(s) on the core-lipid A moiety, rather than on the OPS. Therefore, the epitope specificity and the structures of the O-antigens studied are unique among Proteus serogroups.
【 授权许可】
Unknown
【 预 览 】
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