Structures of the O‐specific polysaccharides and a serological cross‐reactivity of the lipopolysaccharides of Proteus mirabilis O24 and O29

FEBS Letters

Knirel, Yuriy A.²  Senchenkova, Sof'ya N.²  Shashkov, Alexander S.²  Kaca, Wieslaw²  Zatonsky, George V.²  Literacka, Elzbieta¹  Perepelov, Andrei V.² 

[1] Institute of Microbiology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland;N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 117913 Moscow, Russian Federation

关键词: O-antigen;    Lipopolysaccharide;    Bacterial polysaccharide structure;    Serological cross-reactivity;    Proteus mirabilis;   

DOI  :  10.1016/S0014-5793(99)00952-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Strains of Proteus mirabilis belonging to serogroups O24 and O29 are frequent in clinical specimens. Anti-P. mirabilis O24 serum cross-reacted with the lipopolysaccharide (LPS) of P. mirabilis O29 and vice versa. The structures of the O-specific polysaccharides (OPSs, O-antigens) of both LPSs were established using sugar analysis and one- and two-dimensional ¹H- and ¹³C-NMR spectroscopy and found to be different. SDS-PAGE and Western immunoblotting suggested that the serological cross-reactivity of the LPSs is due to a common epitope(s) on the core-lipid A moiety, rather than on the OPS. Therefore, the epitope specificity and the structures of the O-antigens studied are unique among Proteus serogroups.

【 授权许可】

Unknown