Structural and serological studies of the O‐specific polysaccharide of the bacterium Proteus mirabilis O10 containing L‐altruronic acid, a new component of O‐antigens

FEBS Letters

Kaca, W.¹  Knirel, Yuriy A.²  Senchenkova, Sof'ya N.²  Cedzynski, Maciej¹  Paramonov, Nikolay A.²  Shashkov, Alexander S.²  Ziolkowski, Andrzej¹  St. Swierzko, Anna¹  Toukach, Filip V.² 

[1] Center of Microbiology and Virology, Polish Academy of Sciences, ul. Lodowa 106, 93–232 Lodz, Poland;N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 117913 Moscow, Russia

关键词: Lipopolysaccharide serological specificity;    Bacterial polysaccharide structure;    d-Galacturonic acid;    l-Altruronic acid;    Proteus mirabilis;   

DOI  :  10.1016/S0014-5793(96)01061-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An acidic O-specific polysaccharide from the lipopolysaccharide of Proteus mirabilis O10 contains 2-acetamido-2deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, d-galacturonic acid, and l-altruronic acid, the last-named sugar having not been found hitherto in O-antigens. Structure of a branched tetrasaccharide repeating unit of the polysaccharide was established by ¹H and ¹³C NMR spectroscopy, including two-dimensional COSY and rotating-frame NOE spectroscopy. The lateral l-altruronic acid residue plays the immunodominant role in manifestation of the O10 specificity of Proteus, whereas a disaccharide fragment of the main chain in common with the O-specific polysaccharide of P. mirabilis O43 provides the one-way serological cross-reactivity between anti-O10 serum and O43-antigen.

【 授权许可】

Unknown