FEBS Letters | |
Structural and serological studies of the O‐specific polysaccharide of the bacterium Proteus mirabilis O10 containing L‐altruronic acid, a new component of O‐antigens | |
Kaca, W.1  Knirel, Yuriy A.2  Senchenkova, Sof'ya N.2  Cedzynski, Maciej1  Paramonov, Nikolay A.2  Shashkov, Alexander S.2  Ziolkowski, Andrzej1  St. Swierzko, Anna1  Toukach, Filip V.2  | |
[1] Center of Microbiology and Virology, Polish Academy of Sciences, ul. Lodowa 106, 93–232 Lodz, Poland;N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, 117913 Moscow, Russia | |
关键词: Lipopolysaccharide serological specificity; Bacterial polysaccharide structure; d-Galacturonic acid; l-Altruronic acid; Proteus mirabilis; | |
DOI : 10.1016/S0014-5793(96)01061-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
An acidic O-specific polysaccharide from the lipopolysaccharide of Proteus mirabilis O10 contains 2-acetamido-2deoxy-d-glucose, 2-acetamido-2-deoxy-d-galactose, d-galacturonic acid, and l-altruronic acid, the last-named sugar having not been found hitherto in O-antigens. Structure of a branched tetrasaccharide repeating unit of the polysaccharide was established by 1H and 13C NMR spectroscopy, including two-dimensional COSY and rotating-frame NOE spectroscopy. The lateral l-altruronic acid residue plays the immunodominant role in manifestation of the O10 specificity of Proteus, whereas a disaccharide fragment of the main chain in common with the O-specific polysaccharide of P. mirabilis O43 provides the one-way serological cross-reactivity between anti-O10 serum and O43-antigen.
【 授权许可】
Unknown
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