Accommodation of foreign genes into the Sendai virus genome: sizes of inserted genes and viral replication

FEBS Letters

Shioda, Tatsuo¹  Asakawa, Makoto³  Hasegawa, Mamoru³  Nagai, Yoshiyuki⁵  Fukumura, Masayuki³  Kiyotani, Katsuhiro⁴  Tanaka, Akemi²  Kato, Atsushi⁵  Yoshida, Tetsuya⁴  Sakai, Yuko⁵ 

[1] Department of Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan;Department of Pediatrics, Osaka City University Medical School, Osaka, Japan;DNAVEC Research, Tsukuba, Japan;Department of Bacteriology, Hiroshima University School of Medicine, Hiroshima, Japan;Department of Viral Infection, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, Japan

关键词: Sendai virus;    Genome;    Replication;   

DOI  :  10.1016/S0014-5793(99)00960-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sendai virus (SeV) is an enveloped virus with a negative sense genome RNA of about 15.3 kb. We previously established a system to recover an infectious virus entirely from SeV cDNA and illustrated the feasibility of using SeV as a novel expression vector. Here, we have attempted to insert a series of foreign genes into SeV of different lengths to learn how far SeV can accommodate extra genes and how the length of inserted genes affects viral replication in cells cultured in vitro and in the natural host, mice. We show that a gene up to 3.2 kb can be inserted and efficiently expressed and that the replication speed as well as the final virus titers in cell culture are proportionally reduced as the inserted gene length increases. In vivo, such a size-dependent effect was not very clear but a remarkably attenuated replication and pathogenicity were generally seen. Our data further confirmed reinforcement of foreign gene expression in vitro from the V(−) version of SeV in which the accessory V gene had been knocked out. Based on these results, we discuss the utility of SeV vector in terms of both efficiency and safety.

【 授权许可】

Unknown