The X gene of hepatitis B virus shows a high level stimulation of the Rous sarcoma virus long terminal repeat in the methylotropic yeast, Pichia pastoris

FEBS Letters

Kumar, Vijay¹  Lal, Sunil K.¹ 

[1] Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India

关键词: Transactivation by HBV X gene product;    Heterologous gene expression;    Rous sarcoma virus long terminal repeat;    Pichia pastoris;    HBV;    hepatitis B virus;    AOX1 and 2;    alcohol oxidase 1 and 2;    CAT;    chloramphenicol acetyltransferase;    RSV-LTR;    Rous sarcoma virus long terminal repeat;    HBx;    hepatitis B virus X protein;    PAGE;    polyacrylamide gel electrophoresis;    bp;    base-pair;    kbp;    kilobase-pair;   

DOI  :  10.1016/S0014-5793(99)00930-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to study the transactivational property of the X gene in the methylotropic yeast Pichia pastoris, a Rous sarcoma virus-chloramphenicol acetyltransferase (RSV-CAT) cassette was co-transformed and integrated into the host yeast strain as a reporter which showed an overwhelming CAT activity. Immunoprecipitation of the yeast cell extracts with an X-specific monoclonal antibody, however, showed a low level expression of the X gene. Therefore besides a trans-effect of the X protein, the enhanced reporter activity could be a manifestation of a cis-effect of the X gene sequences also. Therefore, unlike the transactivation studies with X gene in animal cells where limited functional activity is observed, P. pastoris appears to be an excellent system to study cis- and trans-aspects of gene regulation by the X gene.

【 授权许可】

Unknown