Expression of heterologous phosphofructokinase genes in yeast

FEBS Letters

Heinisch, Jürgen J.¹ 

[1] Institut für Mikrobiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, Geb.: 26.12, D-40225 Düsseldorf, Germany

关键词: Heterologous gene expression;    Glycolysis;    Saccharomyces cerevisiae;    Galactose induction;   

DOI  :  10.1016/0014-5793(93)80960-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Genes encoding phosphofructokinases (PFK) from Escherichia coli and from the human muscle were expressed in PFK-deficient strains of Saccharomyces cerevisiae under the control of an inducible GAL1 promoter. They restored PFK activity under inducing conditions and complemented the galactose-negative growth phenotype of the recipient strains. The PFK enzymes expressed appear to be stable in yeast. The human muscle enzyme crossreacts with specific antibodies and shows the expected subunit size. As expected, its activity can be activated by fructose-2,6-bisphosphate, in contrast to the bacterial enzyme.

【 授权许可】

Unknown