The sensitivity of versican from rabbit lung to gelatinase A (MMP‐2) and B (MMP‐9) and its involvement in the development of hydraulic lung edema

FEBS Letters

Negrini, Daniela¹  Miserocchi, Giuseppe¹  Albertini, Riccardo²  Passi, Alberto²  De Luca, Giancarlo² 

[1] Institute of Human Physiology, Faculty of Medicine and Surgery, Via Mangiagalli 32, 20133 Milan, Italy;Department of Experimental and Clinical Biomedical Science, University of Insubria, Via Dunant, 21100 Varese, Italy

关键词: Gelatinase;    MMP-2;    MMP-9;    Proteoglycan;    Versican;    Lung edema;    ECM;    extracellular matrix;    MMP;    matrix metalloproteinase;    PG;    proteoglycan;    SDS-PAGE;    sodium dodecyl sulphate-polyacrylamide gel electrophoresis;    GuHCl;    guanidine hydrochloride;    PVDF;    polyvinylidene difluoride;   

DOI  :  10.1016/S0014-5793(99)00929-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Large chondroitinsulphate-containing proteoglycan (versican) isolated from rabbit lung was cleaved by purified gelatinase A (MMP-2) and gelatinase B (MMP-9), as well as by crude enzyme extract from rabbit lung with hydraulic edema. Gelatine zymography, performed after purification of gelatinases by affinity chromatography, demonstrated that the enzyme extract contained two main gelatinolytic bands at about 92 kDa and 72 kDa, identified by specific antisera as the latent proMMP-9 and proMMP-2, respectively. Moreover, enzyme extract from edematous lung showed an increased amount of the proteolytically activated forms of both gelatinases with respect to normal controls. These results suggest that MMP-2 and MMP-9 are involved in the breakdown of versican occurring in rabbit lung during the development of hydraulic edema.

【 授权许可】

Unknown