Activation of MMP‐9 by neutrophil elastase in an in vivo model of acute lung injury

FEBS Letters

Ferry, Gilles³  Lonchampt, Michel²  Canet, Emmanuel²  de Nanteuil, Guillaume¹  Tucker, Gordon C³  Pennel, Laurence² 

[1] Department of Medicinal Chemistry, Institut de Recherches Servier, 11 rue des Moulineaux, 92150 Suresnes, France;Department of Respiratory Pharmacology, Institut de Recherches Servier, 11 rue des Moulineaux, 92150 Suresnes, France;Department of Oncology, Institut de Recherches Servier, 11 rue des Moulineaux, 92150 Suresnes, France

关键词: Acute lung injury;    Elastase;    Gelatinase;    S 18465;    Metalloproteinase;    MMP-9;    Zymography;    ARDS;    acute respiratory distress syndrome;    APMA;    4-aminophenylmercuric acetate;    BAL;    broncho alveolar lavage;    DNP;    2;    4-dinitrophenyl;    ECM;    extracellular matrix;    MMP;    matrix metalloproteinase;    Nma;    N-methylanthranilic acid r(h) recombinant (human);    TIMP;    tissue inhibitor of metalloproteinase;   

DOI  :  10.1016/S0014-5793(96)01508-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of neutrophil elastase on the functional status of gelatinases was studied in an hamster model developed by intratracheal administration of lipopolysaccharide followed by in situ cell activation with phorbol myristate acetate. This resulted in the production in bronchoalveolar lavage fluids, in addition to the matrix metalloproteinase MMP-9, of a 75 kDa gelatinase associated with collagenolytic activity. Treatment in vivo with an elastase inhibitor abolished the latter activity. Since, in addition, elastase activates in vitro purified MMP-9 gelatinase into a similar 75 kDa entity, these data suggest that elastase may be a physiological activator of MMP-9 in vivo.

【 授权许可】

Unknown