Molten globule versus variety of intermediates: influence of anions on pH‐denatured apomyoglobin

FEBS Letters

Tcherkasskaya, Olga¹  Ptitsyn, Oleg B.¹ 

[1] Laboratory of Experimental and Computational Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-5677, USA

关键词: Apomyoglobin;    Fluorescence energy transfer;    Molten globule;    Protein folding;    N;    native state;    MG;    molten globule state;    U;    unfolded state;    apoMb;    apomyoglobin;    Trp;    tryptophan;    Tyr;    tyrosine;   

DOI  :  10.1016/S0014-5793(99)00792-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The molten globule state was shown to be the third thermodynamic state of protein molecules in addition to their native and unfolded states. On the other hand, it was reported that optical and hydrodynamic properties of pH-denatured apomyoglobin depend on the nature of anions added to the protein solution. This observation was used to conclude that there are many ‘partly folded’ intermediates between the native and unfolded states rather than one distinct molten globule state. However, little is known on the structures of pH-denatured apomyoglobin in the presence of different anions. Two tyrosine residues in horse apomyoglobin have been successively modified by the reaction with tetranitromethane. This approach was employed to measure the distances between tryptophans and modified tyrosines in different states of apomyoglobin by the method of direct energy transfer. Experimental data show that the distance between the middle of the A-helix and the beginning of the G-helix and/or the end of the H-helix in ‘anion-induced’ states are very close to those in the native holo- and apomyoglobins. This suggests that the AGH helical complex, being the most structured part of apomyoglobin in the molten globule state, exists also in pH-denatured apomyoglobin in the presence of different anions. Consequently, all non-native forms of apomyoglobin studied so far share the common important feature of its native structure.

【 授权许可】

Unknown