Flow cytometric analysis of intracellular chemokines in chondrocytes in vivo: constitutive expression and enhancement in osteoarthritis and rheumatoid arthritis

FEBS Letters

Facchini, Andrea²  Silvestri, Tania²  Cattini, Luca²  Bassi, Alessandra¹  Macor, Sandro²  Borzı̀, Rosa Maria²  Mazzetti, Ilaria² 

[1] Servizio di Immunoematologia e Medicina Trasfusionale Banca dell'Osso, Istituti Ortopedici Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy;Laboratorio di Immunologia e Genetica, Istituto di Ricerca Codivilla Putti, I.O.R., Via di Barbiano 1/10, 40136 Bologna, Italy

关键词: Chemokine;    Chondrocyte;    Flow cytometry;    Osteoarthritis;    Rheumatoid arthritis;   

DOI  :  10.1016/S0014-5793(99)00886-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment of inflamed joints the intracellular content of CC and CXC chemokines was investigated. IL-8, GROα, MCP-1, RANTES, MIP-1α and MIP-1β expression was evaluated by flow cytometric analysis and RT-PCR in chondrocytes isolated from cartilage specimens obtained from patients with osteoarthritis and rheumatoid arthritis and multiorgan donors as normal controls. All the chemokines except RANTES were found in normal chondrocytes, with different degrees of staining intensity. In osteoarthritis and rheumatoid arthritis patients, an enhancement of IL-8, GROα, MIP-1α and MIP-1β was observed.

【 授权许可】

Unknown