14‐3‐3 proteins control proteolysis of nitrate reductase in spinach leaves

FEBS Letters

Kaiser, Werner M¹  Weiner, Hendrik¹ 

[1] Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl Botanik I, Molekulare Pflanzenphysiologie und Biophysik, Julius-von-Sachs-Platz 2, 97082 Würzburg, Germany

关键词: 14-3-3 protein;    Nitrate reductase;    Protein turnover rate;    Protein:protein interaction;    Protein phosphorylation;   

DOI  :  10.1016/S0014-5793(99)00868-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To test a possible role of 14-3-3 proteins in the degradation of nitrate reductase (NR) in leaves, we monitored 14-3-3s bound to NR in leaf extracts. The amount of 14-3-3s that coimmunoprecipitated with serine 543 phospo-NR (p-NR) increased upon a light/dark transition. This was accompanied by a similar increase in the protein turnover rate of NR in leaves. Purified NR was degraded in extracts from darkened but not from illuminated leaves. Removal of 14-3-3s from such extracts prevented NR degradation. We conclude that the availability of 14-3-3s for p-NR regulates the stability of NR.

【 授权许可】

Unknown