N‐Acetyl‐sphingenine‐1‐phosphate is a potent calcium mobilizing agent

FEBS Letters

Mannaerts, Guy P.¹  Van Veldhoven, Paul P.¹  Gijsbers, Sofie¹  Himpens, Bernard² 

[1] Departement Moleculaire Celbiologie, Afdeling Farmakologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, Herestraat, B-3000 Leuven, Belgium;Departement Moleculaire Celbiologie, Afdeling Fysiologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, Herestraat, B-3000 Leuven, Belgium

关键词: Ceramide-phosphate;    Sphingosine-phosphate;    Lysosphingolipid;    Signal transduction;    Calcium;    CPAE;    calf pulmonary artery endothelial;    fluo-3 AM;    fluo-3 acetoxymethyl ester;    IP3;    inositol-trisphosphate;    HBSS;    Hanks’ buffered salt solution;    N-C2-SeP;    N-acetyl-sphingenine-1-phosphate;    SaP;    sphinganine-1-phosphate;    SeP;    sphingenine-1-phosphate;    SePC;    sphingenine-1-phosphocholine;   

DOI  :  10.1016/S0014-5793(99)00735-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Calcium mobilization induced by phosphorylated sphingoid bases was analyzed in calf pulmonary artery endothelial cells by confocal microscopy. A sphingenine-1-phosphate (SeP) analogue, N-acetyl-sphingenine-1-phosphate (N-C₂-SeP), exogenously added to these cells, caused a fast and transient intracellular rise in calcium and was as potent as SeP. A minimal concentration of 0.6 nM for N-C₂-SeP versus 1 nM for SeP was determined. The N-C₂-SeP-induced Ca²⁺-signaling, like the response to SeP, was due to a release from thapsigargin-sensitive, ryanodine-insensitive, intracellular Ca²⁺-stores and not to a Ca²⁺-influx. N-C₂-SeP can be considered as a truncated ceramide-phosphate, a lipid already reported to be mitogenic (Gomez-Munoz, A., Duffy, P.A., Martin, A., O'Brien, L., Byun, H.S., Bittman, R. and Brindley, D.N. (1995) Mol. Pharmacol. 47, 833–839), an effect that might be secondary to Ca²⁺-mobilization.

【 授权许可】

Unknown