Hypochlorite modification of high density lipoprotein: effects on cholesterol efflux from J774 macrophages

FEBS Letters

Bergt, Constanze¹  Reicher, Helga¹  Malle, Ernst¹  Sattler, Wolfgang¹ 

[1] Institute of Medical Biochemistry, University Graz, Harrachgasse 21, A-8010 Graz, Austria

关键词: Reverse cholesterol transport;    High density lipoprotein modification;    Myeloperoxidase;    Hypochlorite;    Apo;    apolipoprotein;    BSA;    bovine serum albumin;    CE;    cholesteryl ester;    DMEM;    Dulbecco's modified Eagle's medium;    FAME;    fatty acid methyl ester;    FCS;    fetal calf serum;    GC;    gas chromatography;    HDL3;    high density lipoprotein subclass 3;    LPDS;    lipoprotein-deficient serum;    MPO;    myeloperoxidase;    PBS;    phosphate buffered saline;    PL;    phospholipid;    rHDL;    reconstituted HDL;    TBS;    Tris-buffered saline;   

DOI  :  10.1016/S0014-5793(99)00677-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The present study was aimed at investigating effects of hypochlorite (HOCl) modification of high density lipoproteins subclass 3 (HDL₃) on their ability for cellular cholesterol removal from permanent J774 macrophages. Our findings indicate that HOCl (added as reagent or generated enzymatically by the myeloperoxidase/H₂O₂/Cl⁻ system) damages apolipoprotein A-I, the major protein component of HDL₃. Fatty acid analysis of native and HOCl-modified HDL₃ revealed that unsaturated fatty acids in both major lipid subclasses (phospholipids and cholesteryl esters) are targets for HOCl attack. HOCl modification resulted in impaired HDL₃-mediated cholesterol efflux from J774 cells, regardless of whether reagent or enzymatically generated HOCl was used to modify the lipoprotein. Decreased cholesterol efflux was also observed after HOCl modification of reconstituted HDL particles. Impairment of cholesterol efflux from macrophages was noticed at low and physiologically occurring HOCl concentrations.

【 授权许可】

Unknown