Anisoosmotic regulation of the Nopp140 mRNA in H4IIE rat hepatoma cells and primary hepatocytes

FEBS Letters

Häussinger, Dieter¹  Hammermann, Rainer¹  Warskulat, Ulrich¹ 

[1]Medizinische Universitätsklinik der Heinrich-Heine-Universität Düsseldorf, Klinik für Gastroenterologie, Hepatologie und Infektiologie, Moorenstr. 5, D-40225 Düsseldorf, Germany

关键词: Cell volume;    Differential display polymerase chain reaction;    Gene expression;    Liver cell;    Transcription factor;    DDRT-PCR;    differential display polymerase chain reaction;    GAPDH;    glyceraldehyde 3-phosphate dehydrogenase;    MAPK;    mitogen-activated protein kinase;    Nopp140;    nucleolar phosphoprotein of 140 kDa;    PC;    liver parenchymal cells;    PEPCK;    phosphoenolpyruvate carboxykinase;   

DOI  :  10.1016/S0014-5793(99)00643-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using the differential display polymerase chain reaction osmosensitive regulation of mRNA levels of the nucleolar phosphoprotein of 140 kDa (Nopp140) was found in H4IIE rat hepatoma cells. These levels were downregulated after hypoosmotic exposure in H4IIE cells and primary rat hepatocytes. Hyperosmotic incubation increased Nopp140 mRNA levels in H4IIE cells but not in hepatocytes. Inhibition of p38^MAPK or MAP kinase kinase upstream of Erk-1 and Erk-2 decreased Nopp140 mRNA levels but did not prevent their osmosensitivity. Because Nopp140 is involved in the regulation of transcriptional activity it could play a role in the osmosignalling pathway towards gene expression in H4IIE cells and hepatocytes.

【 授权许可】

Unknown