Anisoosmotic regulation of the Mi‐2 autoantigen mRNA in H4IIE rat hepatoma cells and primary hepatocytes

FEBS Letters

Häussinger, Dieter¹  Hammermann, Rainer¹  Warskulat, Ulrich¹ 

[1]Medizinische Universitätsklinik der Heinrich-Heine-Universität Düsseldorf, Klinik für Gastroenterologie, Hepatologie und Infektiologie, Moorenstr. 5, D-40225 Düsseldorf, Germany

关键词: Cell volume;    Differential display polymerase chain reaction;    Gene expression;    Helicase;    Liver cell;    DDRT-PCR;    differential display polymerase chain reaction method;    FBS;    fetal bovine serum;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    PC;    liver parenchymal cells;   

DOI  :  10.1016/S0014-5793(98)01030-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using the differential display polymerase chain reaction (DDRT-PCR) a 169 bp cDNA product, which is 88.8% homologous to the human Mi-2β autoantigen, was identified in H4IIE rat hepatoma cells. At protein level 100% homology was found. The Mi-2 mRNA was downregulated after hypoosmotic exposure and upregulated after hyperosmotic exposure in H4IIE cells and rat hepatocytes. The human Mi-2 is an autoantigen in dermatomyositis and is a member of the SNF/RAD 54 helicase family. Accordingly, Mi-2 may not only be a target of osmosignalling but could also be involved in the osmosignalling pathway towards gene expression in H4IIE and liver parenchymal cells.

【 授权许可】

Unknown