Characterisation of a plant 3‐phosphoinositide‐dependent protein kinase‐1 homologue which contains a pleckstrin homology domain

FEBS Letters

Peter Downes, C.²  Casamayor, Antonio¹  Alessi, Dario R.¹  Currie, Richard A.²  Deak, Maria¹ 

[1] MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, UK;Department of Biochemistry, University of Dundee, Dundee DD1 5EH, UK

关键词: 3-PI;    3-phosphoinositide;    PKB;    protein kinase B;    SGK;    serum and glucocorticoid-regulated protein kinase;    PtdIns;    phosphatidylinositol;    PI 3-kinase;    phosphoinositide 3-kinase;    PH;    pleckstrin homology;   

DOI  :  10.1016/S0014-5793(99)00556-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A plant homologue of mammalian 3-phosphoinositide-dependent protein kinase-1 (PDK1) has been identified in Arabidopsis and rice which displays 40% overall identity with human 3-phosphoinositide-dependent protein kinase-1. Like the mammalian 3-phosphoinositide-dependent protein kinase-1, Arabidopsis 3-phosphoinositide-dependent protein kinase-1 and rice 3-phosphoinositide-dependent protein kinase-1 possess a kinase domain at N-termini and a pleckstrin homology domain at their C-termini. Arabidopsis 3-phosphoinositide-dependent protein kinase-1 can rescue lethality in Saccharomyces cerevisiae caused by disruption of the genes encoding yeast 3-phosphoinositide-dependent protein kinase-1 homologues. Arabidopsis 3-phosphoinositide-dependent protein kinase-1 interacts via its pleckstrin homology domain with phosphatidic acid, PtdIns3P, PtdIns(3,4,5)P₃ and PtdIns(3,4)P₂ and to a lesser extent with PtdIns(4,5)P₂ and PtdIns4P. Arabidopsis 3-phosphoinositide-dependent protein kinase-1 is able to activate human protein kinase Bα (PKB/AKT) in the presence of PtdIns(3,4,5)P₃. Arabidopsis 3-phosphoinositide-dependent protein kinase-1 is only the second plant protein reported to possess a pleckstrin homology domain and the first plant protein shown to bind 3-phosphoinositides.

【 授权许可】

Unknown