Investigation of the interaction of the water oxidising manganese complex of photosystem II with the aqueous solvent environment

FEBS Letters

Evans, Michael C.W¹  Nugent, Jonathan H.A¹  Gourovskaya, Kira¹ 

[1] Dept. of Biology, Darwin Building, University College London, Gower St., London WC1E 6BT, UK

关键词: Photosystem II;    Water oxidation;    Manganese complex;    Electron spin echo envelope modulation;    Photosynthesis;    Photosynthetic oxygen evolution;    EPR;    electron paramagnetic resonance spectroscopy;    ESEEM;    electron spin echo envelope modulation;   

DOI  :  10.1016/S0014-5793(99)00517-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Interaction of the water oxidising manganese complex of photosystem II with the aqueous environment has been investigated using electron paramagnetic resonance spectroscopy and electron spin echo envelope modulation spectroscopy to detect interaction of [²H]methanol with the complex in the S₂ state. The experiments show that the classical S₂ multiline signal is associated with a manganese environment which is not exposed to the aqueous medium. An electron paramagnetic resonance spectroscopy signal, also induced by 200 K illumination, showing ²H modulation by methanol in the medium and a modified multiline electron paramagnetic resonance spectroscopy signal formed in parallel to it, are suggested to be associated with a second manganese environment exposed to the medium.

【 授权许可】

Unknown