Water‐soluble 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin complex with human α‐fetoprotein: properties, toxicity in vivo and antitumor activity in vitro

FEBS Letters

Feldman, Natalya B.¹  Grigor'ev, Michael I.²  Petrov, Rem V.²  Posypanova, Galina A.¹  Severin, Eugene S.²  Sotnichenko, Alexander I.¹  Severin, Sergey E.¹ 

[1]Moscow Research Institute of Medical Ecology, Simferopolsky Blvd. 8, 113149 Moscow, Russia

[2]Center for Medico-Biological and Ecological Research, Simferopolsky Blvd. 8, 113149 Moscow, Russia

关键词: 2;    3;    7;    8-Tetrachlorodibenzo-p-dioxin;    α-Fetoprotein;    Complex;    Solubility;    Toxicity;    Embryotoxicity;    AFP;    human α-fetoprotein;    TCDD;    2;    3;    7;    8-tetrachlorodibenzo-p-dioxin;    FBS;    fetal bovine serum;    HPLC;    high performance liquid chromatography;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(99)00440-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The conditions for the formation of a non-covalent complex between 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and the human transport fetal protein, α-fetoprotein (AFP), have been studied. TCDD has been shown to form a stable complex with AFP in a 2:1 (TCDD:AFP) ratio. The apparent solubility of TCDD in water increases 10⁵-fold after complex formation. The toxicity of the TCDD:AFP complex injected into mice by the intravenous route is comparable with that of free TCDD administered in oil solution per os. The complex manifests very much higher toxicity (200–1400 times) against human tumor cells (CEM, MCF-7, HepG2) in vitro and surpasses TCDD in selectivity. AFP may facilitate TCDD transport in embryonic tissues and enhance its embryotoxic and teratogenic effects.

【 授权许可】

Unknown