Comparison of synthesis and antibacterial activity of temporin A

FEBS Letters

Smoluch, Marek T.²  Harjunpää, Irene³  Silberring, Jerzy²  Kuusela, Pentti⁴  Lankinen, Hilkka³  Wade, David¹ 

[1] Kuwait University, Faculty of Medicine, Department of Biochemistry, P.O. Box 24923, 13110 Safat, Kuwait;Faculty of Chemistry and Regional Laboratory, Jagiellonian University, Ingardena 3, PL-30-060 Krakow, Poland;Haartman Institute, Peptide and Protein Laboratory, Department of Virology, P.O. Box 21, FIN-00014 Helsinki University, Helsinki, Finland;Haartman Institute, Department of Bacteriology and Immunology, FIN-00014 Helsinki University, Helsinki, Finland

关键词: Temporin A;    Synthetic peptide;    2-(4-nitrophenylsulfonyl)ethoxycarbonyl/9-fluorenylmethoxycarbonyl amino acids;    Antibacterial activity;    Staphylococcus aureus;    Enterococcus faecium;   

DOI  :  10.1016/S0014-5793(99)00406-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Temporin A is a small, basic, highly hydrophobic, antibacterial peptide found in the skin of the European red frog, Rana temporaria. It was synthesized twice by the FastMoc solid phase method using amino acids protected at the N^α-position with either 9-fluorenylmethoxycarbonyl or 2-(4-nitrophenylsulfonyl)ethoxycarbonyl. The syntheses of temporin A demonstrates the difference between 2-(4-nitrophenylsulfonyl)ethoxycarbonyl and 9-fluorenylmethoxycarbonyl amino acids. The purified peptide showed also antibacterial activity against clinically important Gram-positive bacteria. It was found to have a moderately good activity against both methicillin resistant and sensitive strains of Staphylococcus aureus, but a weaker activity against vancomycin resistant strains of Enterococcus faecium.

【 授权许可】

Unknown