Human platelets exclusively bind oxidized low density lipoprotein showing no specificity for acetylated low density lipoprotein

FEBS Letters

Volf, I¹  Schmid, W¹  Moeslinger, T¹  Cooper, J¹  Koller, E¹ 

[1]Institute of Medical Physiology, Schwarzspanierstr. 17, A-1090 Vienna, Austria

关键词: CD36;    Oxidized low density lipoprotein;    Scavenger receptor;    Platelet;    AcLDL;    acetylated LDL;    OxLDL;    oxidized LDL;    PBS;    phosphate-buffered saline;    LDL;    low density lipoprotein;    M-BSA;    maleylated bovine serum albumin;    mAb;    monoclonal antibody;    SR;    scavenger receptor;    EDTA;    ethylenediaminetetraacetic acid;    FCS;    fetal calf serum;    f.c.;    final concentration;   

DOI  :  10.1016/S0014-5793(99)00437-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The widely studied macrophage scavenger receptor system is known to bind both acetylated low density lipoprotein and oxidized low density lipoprotein. Although only the latter ligand has been shown to occur in vivo, acetylated low density lipoprotein is often used to evaluate the contribution of scavenger receptors to different (patho)physiologic processes, assuming that all existing subtypes of scavenger receptors recognise both lipoproteins. In the present work, we identify human platelets as the first natural cell type to bind oxidized low density lipoprotein without showing specificity for acetylated low density lipoprotein. Consequently, platelets possess exclusive receptor(s) for oxidized low density lipoprotein distinct from the ‘classical’ scavenger receptor AI/AII. From the data presented in this work, we conclude that the class B scavenger receptor CD36 (GPIV) is responsible for this exclusive oxidized low density lipoprotein binding.

【 授权许可】

Unknown