Functional and possible physical association of scavenger receptor with cytoplasmic tyrosine kinase Lyn in monocytic THP‐1‐derived macrophages

FEBS Letters

Hojo, Hironobu⁵  Miki, Yoshitsugu⁶  Nakamura, Yu³  Miki, Shunji⁴  Aimoto, Saburo¹  Sato, Bunzo⁴  Tsukada, Satoshi²  Yamamoto, Masahiro⁴ 

[1] Institute of Protein Research, Osaka University, Osaka, Japan;Department of Internal Medicine III, Osaka University, 2-2, Yamadaoka, Suita-city, Osaka 565, Japan;Department of Neuropsychiatry, Osaka University, Osaka, Japan;Nissei Hospital, 6-3-8, Itachibori, Nishiku, Osaka 550, Japan;Department of Bioapplied Chemistry, Faculty of Engineering, Osaka City University, Osaka, Japan;Miki Clinic, 2-2-1, Kuzuhara, Neyagawashi, Osaka 572, Japan

关键词: THP-1-derived macrophage;    Lyn;    Scavenger receptor;    Acetyl low-density lipoprotein;   

DOI  :  10.1016/S0014-5793(96)01332-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Acetyl LDL (modified low-density lipoprotein), which is thought to be taken up through scavenger receptor A (SR-A), rapidly induced the appearance of phosphotyrosine proteins in monocytic THP-1-derived macrophages in vitro. The two alternative forms of Lyn (p⁵³ and p⁵⁶) were found to be tyrosine-phosphorylated within 30 s after the stimulation with acetyl LDL. The catalytic activity of Lyn measured by an in vitro kinase assay had also increased in acetyl LDL-stimulated THP-1-derived macrophages. Furthermore, Lyn could be co-immunoprecipitated with SR-A from the cell lysate. These observations suggest a functional and possible physical association of SR-A with Lyn in THP-1-derived macrophages, and also imply a possible involvement of Lyn in SR-A signal transduction.

【 授权许可】

Unknown