The unique N‐terminal domain of the cAMP phosphodiesterase PDE4D4 allows for interaction with specific SH3 domains

FEBS Letters

Beard, Matthew B.¹  Bolger, Graeme B.²  Houslay, Miles D.¹  O'Connell, Jonathan C.¹ 

[1] Molecular Pharmacology Group, Division of Biochemistry and Molecular Biology, Davidson and Wolfson Buildings, IBLS, University of Glasgow, Glasgow G12 8QQ, UK;Veterans Affairs Medical Center, Departments of Medicine (Dvn. Oncology) and Oncological Science, University of Utah, 500 Foothill Blvd., Salt Lake City, UT 84148, USA

关键词: cAMP phosphodiesterase;    Src homology 3 domain;    Src family tyrosyl kinase;    Lyn;    Protein-protein interaction;    Compartmentalisation;    PDE;    cyclic nucleotide phosphodiesterase;    UCR;    upstream conserved region;    SH3;    src homology 3 domain;   

DOI  :  10.1016/S0014-5793(99)01335-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Of the five PDE4D isoenzymes, only the PDE4D4 cAMP specific phosphodiesterase was able to bind to SH3 domains. Only PDE4D4 and PDE4A5, but not any other PDE4A, B, C and D isoforms expressed in rat brain, bound to src, lyn and fyn kinase SH3 domains. Purified PDE4D4 could bind to purified lyn SH3. PDE4D4 and PDE4A5 both exhibited selectivity for binding the SH3 domains of certain proteins. PDE4D4 did not bind to WW domains. We suggest that an important function of the unique N-terminal region of PDE4D4 may be to allow for association with certain SH3 domain-containing proteins.

【 授权许可】

Unknown