A critical comparison of the hemolytic and fungicidal activities of cationic antimicrobial peptides

FEBS Letters

van 't Hof, Wim¹  Nieuw Amerongen, Arie V¹  Veerman, Enno C.I¹  Reijnders, Ingrid M¹  Helmerhorst, Eva J¹ 

[1] Academic Centre for Dentistry Amsterdam (ACTA), Department of Oral Biochemistry, Vrije Universiteit, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands

关键词: Hemolytic activity;    Fungicidal activity;    Cationic peptide;    Antimicrobial;   

DOI  :  10.1016/S0014-5793(99)00411-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hemolytic and fungicidal activity of a number of cationic antimicrobial peptides was investigated. Histatins and magainins were inactive against human erythrocytes and Candida albicans cells in phosphate buffered saline, but displayed strong activity against both cell types when tested in 1 mM potassium phosphate buffer supplemented with 287 mM glucose. The HC₅₀/IC₅₀ ratio, indicative of the therapeutic index, was about 30 for all peptides tested. PGLa was most hemolytic (HC₅₀=0.6 μM) and had the lowest therapeutic index (HC₅₀/IC₅₀=0.5). Susceptibility to hemolysis was shown to increase with storage duration of the erythrocytes and also significant differences were found between blood collected from different individuals. In this report, a sensitive assay is proposed for the testing of the hemolytic activities of cationic peptides. This assay detects subtle differences between peptides and allows the comparison between the hemolytic and fungicidal potency of cationic peptides.

【 授权许可】

Unknown