FEBS Letters | |
Characterization of the promoters of the guinea pig neutrophil cationic peptide‐1 and ‐2 genes | |
Nagaoka, Isao1  Yamashita, Tatsuhisa1  Ishihara, Noriko1  | |
[1] Department of Biochemistry, Juntendo University, School of Medicine, Hongo, Bunkyo-ku, Tokyo 113, Japan | |
关键词: Cationic peptide; Defensin; Gene expression; Promoter; Neutrophil; Guinea pig; GNCP; guinea pig neutrophil cationic peptide; PCR; polymerase chain reaction; HEPES; 4-(2-hydroxyethyl)-piperazineethanesulfonic acid; PMSF; phenylmethylsulfonyl fluoride; kb; kilo-base; bp; base pair; | |
DOI : 10.1016/0014-5793(94)01229-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Guinea pig neutrophils contain the antimicrobial cationic peptides GNCP-1 and GNCP-2 in the granules. To understand the regulation of the gene expression, the promoters for the GNCP-1 and GNCP-2 genes were characterized. Sequencing analysis of the genomic clones showed that the nucleotide sequences of the 5′-upstream regions (1.7 kb) from exon 1 were homologous (90–93%) between the GNCP-1 and GNCP-2 genes. However, transient transfection assays using luciferase reporter gene constructs revealed that the promoter activity of GNCP-1 was 2-fold greater than that of GNCP-2. Furthermore, DNase I footprint analysis demonstrated that three regions (I, II and III) were protected on the GNCP-1 promoter, whereas only two protected regions (II and III) were identified on the GNCP-2 promoter. Together these observations indicate that GNCP-1 and GNCP-2 are encoded by homologous genes, but the expression of the GNCP-1 and GNCP-2 genes is likely to be different at the level of transcription.
【 授权许可】
Unknown
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