Characterization of the promoters of the guinea pig neutrophil cationic peptide‐1 and ‐2 genes

FEBS Letters

Nagaoka, Isao¹  Yamashita, Tatsuhisa¹  Ishihara, Noriko¹ 

[1] Department of Biochemistry, Juntendo University, School of Medicine, Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: Cationic peptide;    Defensin;    Gene expression;    Promoter;    Neutrophil;    Guinea pig;    GNCP;    guinea pig neutrophil cationic peptide;    PCR;    polymerase chain reaction;    HEPES;    4-(2-hydroxyethyl)-piperazineethanesulfonic acid;    PMSF;    phenylmethylsulfonyl fluoride;    kb;    kilo-base;    bp;    base pair;   

DOI  :  10.1016/0014-5793(94)01229-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Guinea pig neutrophils contain the antimicrobial cationic peptides GNCP-1 and GNCP-2 in the granules. To understand the regulation of the gene expression, the promoters for the GNCP-1 and GNCP-2 genes were characterized. Sequencing analysis of the genomic clones showed that the nucleotide sequences of the 5′-upstream regions (1.7 kb) from exon 1 were homologous (90–93%) between the GNCP-1 and GNCP-2 genes. However, transient transfection assays using luciferase reporter gene constructs revealed that the promoter activity of GNCP-1 was 2-fold greater than that of GNCP-2. Furthermore, DNase I footprint analysis demonstrated that three regions (I, II and III) were protected on the GNCP-1 promoter, whereas only two protected regions (II and III) were identified on the GNCP-2 promoter. Together these observations indicate that GNCP-1 and GNCP-2 are encoded by homologous genes, but the expression of the GNCP-1 and GNCP-2 genes is likely to be different at the level of transcription.

【 授权许可】

Unknown