Isolation of cDNA clones encoding two isoforms of nucleoside diphosphate kinase from bovine retina

FEBS Letters

Kakuev, Dmitry¹  Karaschuk, Galina¹ 

[1] Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10, Miklukho-Maklaya, 117871 Moscow GSP-7 V-437, Russia

关键词: Nucleoside diphosphate kinase;    Bovine retina;    cDNA cloning;   

DOI  :  10.1016/S0014-5793(99)00349-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cDNA encoding bovine retinal isoforms of nucleoside diphosphate kinase (NDP-kinase, EC 2.7.4.6) has been cloned and sequenced. Based on the partial amino acid sequence of the enzyme determined after trypsin digestion of purified NDP-kinase, primers were synthesized and used to isolate two different cDNA clones encoding the full length of two NDP-kinase isoforms. The nucleotide sequences of these clones contained open reading frames encoding 152-residue polypeptides with calculated molecular masses of 17.262 and 17.299 kDa, similar to that determined for the subunits of purified enzyme (17.5 and 18.5 kDa). The deduced NDP-kinase sequences showed high similarity with the known NDP-kinase sequences from other sources.

【 授权许可】

Unknown