Inhibition of nucleoside diphosphate kinase (NDPK/nm23) by cAMP analogues

FEBS Letters

Van Dommelen, Kristof¹  Anciaux, Katelijne¹  Roymans, Dirk¹  Willems, Roel¹  Slegers, Herman¹ 

[1] Cellular Biochemistry, Department of Biochemistry, Universitaire Instelling Antwerpen, Universiteitsplein 1, B-2610 Wilrijk-Antwerpen, Belgium

关键词: cAMP;    8-Chloro cAMP;    Nucleoside diphosphate kinase;    nm23;    Breast cancer;    8-BrcAMP;    8-bromo cAMP;    8-ClcAMP;    8-chloro cAMP;    dbcAMP;    N 6;    O′2-dibutyryl cAMP;    IGF;    insulin-like growth factor;    N 6-mbcAMP;    N 6-monobutyryl cAMP;    O′2-mbcAMP;    O′2-monobutyryl cAMP;    PDGF;    platelet-derived growth factor;    TLC;    thin-layer chromatography;   

DOI  :  10.1016/S0014-5793(96)01358-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nucleoside diphosphate kinase (NDPK/nm23) ATP/GDP phosphotransferase activity and serine autophosphorylation is inhibited by N ⁶-mbcAMP, 8-ClcAMP and 8-BrcAMP. Inhibition of the enzymatic activity largely depends on the concentration of ATP and becomes significant at ATP concentrations up to 0.5 mM and at effector concentrations measured in C6 cells stimulated with 1 mM cAMP analogue. N ⁶-mbcAMP is a substrate of the enzyme. DbcAMP and O′²-mbcAMP, cAMP analogues with a modified O′²-ribose, did not affect the NDPK activity. Cyclic AMP is only a moderate inhibitor of NDPK even at low ATP concentrations. Possible inhibitory effects of cAMP and cAMP analogues on reported extra- and intracellular functions of NDPK/nm23 are discussed.

【 授权许可】

Unknown