A new human nm23 homologue (nm23‐H5) specifically expressed in testis germinal cells

FEBS Letters

Feral, Chloé²  Munier, Annie¹  Lacombe, Marie-Lise¹  Gyapay, Gabor³  Capeau, Jacqueline¹  Milon, Laurence¹  Pinon, Véronique Phung-Ba¹  Guellaen, Georges² 

[1] INSERM U402, Faculté de Médecine Saint-Antoine, Paris, France;INSERM U99, Hôpital Henri Mondor, Créteil, France;CNRS URA 1922, Genethon, Evry, France

关键词: Nucleoside diphosphate kinase;    nm23;    Testis;    Metastasis;    Cancer;   

DOI  :  10.1016/S0014-5793(98)00996-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified a cDNA encoding a 212 amino acid protein (Nm23-H5) with 27–31% identity to the human members of the nm23/nucleoside diphosphate (NDP) kinase gene family. The nm23-H5 gene is located on chromosome 5q23–31 and is transcribed as one main transcript of 1.1 kb which is highly and specifically expressed in testis, in the spermatogonia and early spermatocytes. Nm23-H5 possesses most of the residues conserved among NDP kinases plus an additional COOH-terminus end of 55 amino acids unique to this protein. However, under usual assay conditions, Nm23-H5 expressed in Escherichia coli did not exhibit NDP kinase activity. These results suggest that Nm23-H5 is specifically involved in early stages of spermatogenesis.

【 授权许可】

Unknown