FEBS Letters | |
Evidence for peroxynitrite formation during S‐nitrosoglutathione photolysis in air saturated solutions | |
Akhter, Shirin2  Redmond, Robert W1  Mutus, Bulent2  | |
[1] Wellman Laboratories of Photomedicine, Department of Dermatology, Harvard Medical School, Masssachusetts General Hospital, Boston, MA 02165, USA;Chemistry and Biochemistry, School of Physical Sciences, University of Windsor, Windsor, Ont. N9B 3P4, Canada | |
关键词: S-Nitrosoglutathione; Peroxynitrite; Photolysis; Nitric oxide; | |
DOI : 10.1016/S0014-5793(99)00311-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Flash photolysis of S-nitrosoglutathione (GSNO) in aerated solutions at pH 10 gave rise to an absorption with a maximum around 310–320 nm. This peak is spectrally similar to that displayed by ONOO−. The decay kinetics of this absorption was compared to that of authentic ONOO−, generated independently. An excellent correlation was obtained. Further proof of ONOO− generation was provided by HPLC studies showing the production of 3-nitrotyrosine on irradiation of GSNO in the presence of tyrosine at pH 7.4. In addition, the nitration yield was increased ∼5-fold in the presence of bicarbonate and totally eliminated with DMPO, indicating the requirement of a radical intermediate for peroxynitrite production during S-nitrosothiol photolysis.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020307578ZK.pdf | 171KB | download |