Evidence for peroxynitrite formation during S‐nitrosoglutathione photolysis in air saturated solutions

FEBS Letters

Akhter, Shirin²  Redmond, Robert W¹  Mutus, Bulent² 

[1] Wellman Laboratories of Photomedicine, Department of Dermatology, Harvard Medical School, Masssachusetts General Hospital, Boston, MA 02165, USA;Chemistry and Biochemistry, School of Physical Sciences, University of Windsor, Windsor, Ont. N9B 3P4, Canada

关键词: S-Nitrosoglutathione;    Peroxynitrite;    Photolysis;    Nitric oxide;   

DOI  :  10.1016/S0014-5793(99)00311-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Flash photolysis of S-nitrosoglutathione (GSNO) in aerated solutions at pH 10 gave rise to an absorption with a maximum around 310–320 nm. This peak is spectrally similar to that displayed by ONOO⁻. The decay kinetics of this absorption was compared to that of authentic ONOO⁻, generated independently. An excellent correlation was obtained. Further proof of ONOO⁻ generation was provided by HPLC studies showing the production of 3-nitrotyrosine on irradiation of GSNO in the presence of tyrosine at pH 7.4. In addition, the nitration yield was increased ∼5-fold in the presence of bicarbonate and totally eliminated with DMPO, indicating the requirement of a radical intermediate for peroxynitrite production during S-nitrosothiol photolysis.

【 授权许可】

Unknown