Cell cycle arrest mediated by hepatitis delta antigen

FEBS Letters

Park, Kyu-Jin¹  Hwang, Soon B¹ 

[1] Institute of Environment and Life Science, The Hallym Academy of Sciences, Hallym University, Chuncheon 200-702, South Korea

关键词: Cell cycle arrest;    Flow cytometry;    Hepatitis;    delta antigen;    Baculovirus;    Hepatitis delta virus;    Spodoptera frugiperda;   

DOI  :  10.1016/S0014-5793(99)00394-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hepatitis delta antigen (HDAg) is the only viral-encoded protein of the hepatitis delta virus (HDV). This protein has been extensively characterized with respect to its biochemical and functional properties. However, the molecular mechanism responsible for persistent HDV infection is not yet clear. Previously, we reported that overexpression of HDAg protects insect cells from baculovirus-induced cytolysis [Hwang, S.B. Park, K.-J. and Kim, Y.S. (1998) Biochem. Biophys. Res. Commun. 244, 652–658]. Here we report that HDAg mediates cell cycle arrest when overexpressed in recombinant baculovirus-infected insect cells. Flow cytometry analysis has shown that HDAg expression in Spodoptera frugiperda cells causes an accumulation of substantial amounts of polyploid DNA in the absence of cell division. This phenomenon may be partly responsible for the persistent infection of chronic HDV patients.

【 授权许可】

Unknown