Ultraviolet‐B induction of interstitial collagenase and stromelyin‐1 occurs in human dermal fibroblasts via an autocrine interleukin‐6‐dependent loop

FEBS Letters

Hinrichs, Ralf¹  Krieg, Thomas¹  Brenneisen, Peter¹  Wlaschek, Meinhard¹  Dissemond, Joachim¹  Scharffetter-Kochanek, Karin¹  Wenk, Jutta¹  Blaudschun, Ralf¹ 

[1] Department of Dermatology, University of Cologne, Joseph-Stelzmann Str. 9, D-50924 Cologne, Germany

关键词: Ultraviolet-B;    Interleukin-6;    Collagenase;    Stromelysin-1;    Autocrine mechanism;    Fibroblast;    DMEM;    Dulbecco's modified Eagle's medium;    ELISA;    enzyme-linked immunosorbent assay;    FCS;    fetal calf serum;    IL;    interleukin;    MMP;    matrix-degrading metalloproteinase;    PBS;    phosphate-buffered saline;    ROS;    reactive oxygen species;    TIMP;    tissue inhibitor of matrix-degrading metalloproteinases;    TMB;    3;    3′;    5;    5′-tetramethylbenzidine;    TNF;    tumor necrosis factor;    UV;    ultraviolet;   

DOI  :  10.1016/S0014-5793(99)00402-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ultraviolet-B irradiation of human dermal fibroblasts has earlier been shown to induce matrix-degrading metalloproteinases, thus driving connective tissue degradation in photoaging and photocarcinogenesis. Herein, we report that Ultraviolet-B irradiation led to a dramatic increase in specific mRNA and protein levels of interstitial collagenase, stromelysin and interleukin-6. By contrast, the major tissue inhibitor of matrix-degrading metalloproteinases, TIMP-1, was unaffected. Monospecific neutralizing antibodies directed against human interleukin-6 significantly reduced the interstitial collagenase and stromelysin-1 protein levels. Taken together, our data provide the first evidence that Ultraviolet-B induction of interstitial collagenase and stromelysin-1 occurs via the synthesis and release of interleukin-6. Hence, this newly identified autocrine mechanism may contribute to dermal photodamage.

【 授权许可】

Unknown