FEBS Letters | |
A potential NES of the Epstein‐Barr virus nuclear antigen 1 (EBNA1) does not confer shuttling | |
Grässer, Friedrich A.1  Voß, Marc D.1  Fischer, Nicole1  Mueller-Lantzsch, Nikolaus1  | |
[1] Institut für Mikrobiologie und Hygiene, Abteilung Virologie, Haus 47, Universitätskliniken, D-66421 Homburg/Saar, Germany | |
关键词: EBNA1; Nuclear export; Heterokaryon assay; | |
DOI : 10.1016/S0014-5793(99)00313-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The Epstein-Barr virus nuclear antigen 1 (EBNA1) is a multifunctional protein involved in the replication and maintenance of the viral episome. We identified a potential Rev-like nuclear export signal (NES) which, however, does not confer the export of EBNA1. In the yeast two-hybrid system EBNA1 does not bind to the nuclear exporter Crm1p. In spite of the RNA-binding ability of EBNA1 and its structural homologies to RNA binding proteins like hnRNP U and/or A1, EBNA1 does not shuttle to the cytoplasm in heterokaryon analysis. We propose the function of the RNA binding of EBNA1 in retaining RNAs to the nucleus.
【 授权许可】
Unknown
【 预 览 】
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