A potential NES of the Epstein‐Barr virus nuclear antigen 1 (EBNA1) does not confer shuttling

FEBS Letters

Grässer, Friedrich A.¹  Voß, Marc D.¹  Fischer, Nicole¹  Mueller-Lantzsch, Nikolaus¹ 

[1] Institut für Mikrobiologie und Hygiene, Abteilung Virologie, Haus 47, Universitätskliniken, D-66421 Homburg/Saar, Germany

关键词: EBNA1;    Nuclear export;    Heterokaryon assay;   

DOI  :  10.1016/S0014-5793(99)00313-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Epstein-Barr virus nuclear antigen 1 (EBNA1) is a multifunctional protein involved in the replication and maintenance of the viral episome. We identified a potential Rev-like nuclear export signal (NES) which, however, does not confer the export of EBNA1. In the yeast two-hybrid system EBNA1 does not bind to the nuclear exporter Crm1p. In spite of the RNA-binding ability of EBNA1 and its structural homologies to RNA binding proteins like hnRNP U and/or A1, EBNA1 does not shuttle to the cytoplasm in heterokaryon analysis. We propose the function of the RNA binding of EBNA1 in retaining RNAs to the nucleus.

【 授权许可】

Unknown