The Epstein–Barr virus nuclear antigen‐1 may act as a transforming suppressor of the HER2/neu oncogene

FEBS Letters

Law, Sai-Lung³  Kao, Ming-Ching¹  Way, Tzong-Der²  Chuang, Tzu-Chao¹  Lee, Ying-Chu²  Lin, Yen-Shing² 

[1] Graduate Institute of Life Sciences, National Defense Medical Center, National Defense University, Taipei, Taiwan;Department of Biochemistry, National Defense Medical Center, National Defense University, Neihu, P.O. Box 90048-501, Taipei, Taiwan;Pharmaceutics Research Laboratory, Department of Medical Research and Education, Veterans General Hospital, Taipei 114, Taiwan

关键词: HER2/neu;    EBNA1;    Tumorigenicity;    Ovarian cancer;   

DOI  :  10.1016/S0014-5793(02)03658-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It is known that the HER2/neu proto-oncogene is associated with a wide variety of human cancers and considered to be an attractive target for developing anti-cancer agents. We report here for the first time that the Epstein–Barr virus nuclear antigen-1 (EBNA1) suppresses the HER2/neu oncogene expression at the transcriptional level. Recombinant clones of EBNA1 were subcloned and stably transfected into HER2/neu-overexpressing human ovarian cancer SKOV3.ip1 cells. These EBNA1-containing clones down-regulated the endogenous production of p185 ^HER2/neu . In addition, the EBNA1-expressing stable transfectants showed reduced growth rate, low soft agarose colony-forming ability and tumorigenic potential as compared with the parental line. These data suggest that EBNA1 may act as a transforming suppressor of the HER2/neu oncogene.

【 授权许可】

Unknown