Thioredoxin peroxidase in the Cyanobacterium Synechocystis sp. PCC 6803

FEBS Letters

Dietz, Karl-Josef³  Yokota, Akiho¹  Miyake, Chikahiro²  Murata, Norio⁴  Yamamoto, Hiroshi¹  Tomizawa, Ken-Ichi¹ 

[1] Plant Molecular Physiology Laboratory, Research Institute of Innovative Technology for the Earth (RITE), Kizu, Kyoto 619-0292, Japan;Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama, Ikoma, Nara 630-0101, Japan;Lehrstuhl für Stoffwechselphysiologie und Biochemie der Pflanzen, Fakultät für Biologie W5, Universität Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany;Department of Molecular Biomechanics, Graduate University of Advanced Studies, Myodaiji, Okazaki 444-8585, Japan

关键词: Chlorophyll fluorescence;    Cyanobacterium;    Hydrogen peroxide;    Thioredoxin peroxidase;    Synechocystis sp. PCC 6803;    AOS;    active oxygen species;    DCMU;    3-(3;    4 dichlorophenyl)-1;    1-dimethylurea;    DTT;    dithiothreitol;    IPTG;    isopropyl-d-thiogalactopyranoside;    MeSH;    2-mercaptoethanol;    ORF;    open reading frame;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    t-BuOOH;    tertiary butyl hydroperoxide;    TPX;    thioredoxin peroxidase;   

DOI  :  10.1016/S0014-5793(99)00309-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The amino acid sequence deduced from the open reading frame designated sll0755 in Synechocystis sp. PCC 6803 is similar to the amino acid sequences of thioredoxin peroxidases from other organisms. In the present study, we found that a recombinant SLL0755 protein that was expressed in Escherichia coli was able to reduce H₂O₂ and tertiary butyl hydroperoxide with thioredoxin from E. coli as the electron donor. Targeted disruption of open reading frame sll0755 in Synechocystis sp. PCC 6803 cells completely eliminated the H₂O₂-dependent and tertiary butyl hydroperoxide-dependent photosynthetic evolution of oxygen and the electron flow in photosystem II. These results indicate that the product of open reading frame sll0755 is a thioredoxin peroxidase whose activities are coupled to the photosynthetic electron transport system in Synechocystis sp. PCC 6803.

【 授权许可】

Unknown