Hypothetical protein KIAA0079 is a mammalian homologue of yeast Sec24p

FEBS Letters

Tani, Katsuko¹  Hatsuzawa, Kiyotaka¹  Tagaya, Mitsuo¹  Oyama, Yuki¹ 

[1]School of Life Science, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo 192-0392, Japan

关键词: Sec23p;    Sec24p;    Coat protein II;    Endoplasmic reticulum;    Vesicular transport;    COP;    coat protein;    ER;    endoplasmic reticulum;    GST;    glutathione S-transferase;    GFP;    green fluorescent protein;    mSec23p;    mouse Sec23p;    PAGE;    polyacrylamide gel electrophoresis;    VSVG;    vesicular stomatitis virus-encoded glycoprotein;   

DOI  :  10.1016/S0014-5793(99)00303-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Sec23p-Sec24p complex is a component of COPII-coated vesicles that mediate protein transport from the endoplasmic reticulum in yeast. The mammalian hypothetical protein KIAA0079 (KIAA0079p) exhibits sequence similarity to yeast Sec24p. KIAA0079p was co-eluted with mammalian Sec23p on gel filtration. In vitro binding experiments revealed that the C-terminal region of KIAA0079p binds to the N-terminal region of mammalian Sec23p. Overexpression of KIAA0079p caused a defect in protein export from the endoplasmic reticulum. These results support the idea that KIAA0079p is a functional homologue of yeast Sec24p.

【 授权许可】

Unknown