期刊论文详细信息
FEBS Letters | |
DNA triple helix stabilisation by a naphthylquinoline dimer | |
Keppler, Melanie1  Zegrocka, Oliwia1  Fox, Keith R1  Strekowski, Lucjan2  | |
[1] Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK;Department of Chemistry, Georgia State University, University Plaza, Atlanta, GA 30303, USA | |
关键词: Triplex ligand; Footprinting; DNA triplex; Bis-intercalator; Naphthylquinoline; | |
DOI : 10.1016/S0014-5793(99)00284-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9-mer oligonucleotide 5′-TTTTTTCTT and its oligopurine duplex target at concentrations as low as 0.1 μM, enhancing the triplex stability by at least 1000-fold. This compound, which is the first reported example of a triplex bis-intercalator, is about 30 times more potent than the simple monofunctional ligand.
【 授权许可】
Unknown
【 预 览 】
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RO201912020307470ZK.pdf | 225KB | download |