DNA triple helix stabilisation by a naphthylquinoline dimer

FEBS Letters

Keppler, Melanie¹  Zegrocka, Oliwia¹  Fox, Keith R¹  Strekowski, Lucjan² 

[1] Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, UK;Department of Chemistry, Georgia State University, University Plaza, Atlanta, GA 30303, USA

关键词: Triplex ligand;    Footprinting;    DNA triplex;    Bis-intercalator;    Naphthylquinoline;   

DOI  :  10.1016/S0014-5793(99)00284-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9-mer oligonucleotide 5′-TTTTTTCTT and its oligopurine duplex target at concentrations as low as 0.1 μM, enhancing the triplex stability by at least 1000-fold. This compound, which is the first reported example of a triplex bis-intercalator, is about 30 times more potent than the simple monofunctional ligand.

【 授权许可】

Unknown